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The B-Cell Population Produces Antibodies to Millions of Different AntigensfYet the Antibody-Antigen Interaction Is Specific

Antibodies bind to antigen through their antigen-binding sites. Each antigen-binding site is formed by the steric interaction of the V∣ and V∣∣ domains, which come in close contact because of the three-dimensional folding of the glyco­protein chains on which they reside.

This steric interaction essentially forms a cleft, and any antigenic structure that fits into this cleft is recognized and binds to the antigen-binding site. Therefore, if an antigen can bind to the antigen-com­bining site on the BCR, the B cell is eventually (after a com­plex set of signal interactions) triggered to replicate (clonally expand), giving rise to many uidenticaΓ,B cells, which even­tually produce and secrete the soluble immunoglobulin specific for that antigen.

It is important to stress that antibody-antigen responses are specific. Antibodies produced after exposure to an antigen will only bind to that antigen or to other antigens structurally similar to the original antigen (cross-reaction). The question is, mHow does the immune system manage to respond to liter­ally several million different foreign antigens in a specific way?” Three facts are crucial to understand this situation. First, the BCR is an immunoglobulin, and the specificity of the immunoglobulin secreted by a particular B cell is the same as the specificity of its BCR. Second, an individual B cell can only have BCRs of identical specificity. Third, essentially each B cell (and there are millions) in the body has a BCR with a different antigen-combining site, because B cells undergo random genetic mutations in the genes coding for their Vl and Vh domains during their early development (ontogeny). Because these domains make up the antigen-combining site, a single amino acid change in either of the regions changes the steric interaction of these domains, giving rise to different “clefts” with different antigen-combining abilities.

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Source: Cunningham J.G., Klein B.G.. Textbook of Veterinary Physiology. Elsevier Health Sciences,2007. — 720 ð.. 2007

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